c33a cells  (ATCC)

Journal: Current Issues in Molecular Biology

Article Title: The Therapeutic Effect of EZH2 Inhibitors in Targeting Human Papillomavirus Associated Cervical Cancer

doi: 10.3390/cimb47120990

Figure Lengend Snippet: MTT assay results for cytotoxicity of drug treatments. Drug dose–response curves after 48 h for HPV- C33a, HPV+ CaSki, and normal epithelial cell line CRL1790: ( A ) cisplatin, ( B ) EPZ6438, and ( C ) ZLD1039. Data is expressed as log of mean ± SD (n = 3). IC50 values, pinpointed on the graph, were calculated using GraphPad prism. ( D ) Table of mean IC50 value data (n = 3). The IC50 value for EPZ6438 could not be determined within the 0–80 µM range.

Article Snippet: According to human protein atlas [ ], the C33a cell line exhibits no expression of CDH1 gene, which encodes E-cadherin; however, mass spectrometry proteomics data could further confirm its protein expression.

Techniques: MTT Assay

Journal: Current Issues in Molecular Biology

Article Title: The Therapeutic Effect of EZH2 Inhibitors in Targeting Human Papillomavirus Associated Cervical Cancer

doi: 10.3390/cimb47120990

Figure Lengend Snippet: Migration rate assessment following the scratch wound–healing assay. ( A ) Representative images from the in vitro scratch wound–healing assays demonstrating cell migration into the cell-free region (outlined by black lines) following 48 h treatment (scale = 100 µm). ( B ) Summary plots showing the migration rates by C33a and CaSki cells after treatment (Mean ± SD, n = 3).

Article Snippet: According to human protein atlas [ ], the C33a cell line exhibits no expression of CDH1 gene, which encodes E-cadherin; however, mass spectrometry proteomics data could further confirm its protein expression.

Techniques: Migration, Wound Healing Assay, In Vitro

Journal: Current Issues in Molecular Biology

Article Title: The Therapeutic Effect of EZH2 Inhibitors in Targeting Human Papillomavirus Associated Cervical Cancer

doi: 10.3390/cimb47120990

Figure Lengend Snippet: Effect of EZH2 inhibitors on protein and mRNA expression of ZO-1 and E-cadherin on cervical cancer cells after 48 h treatment. ( A ) Immunofluorescence staining of ZO-1 and E-cadherin protein expression (pink) and nuclei (blue) under 400× microscope magnification. Scale = 50 µm. Mean fluorescence intensity of immunocytochemistry results were normalised to control cells (right) (n = 3). ( B ) Representative Western blots’ results of protein bands ZO-1 (250 kDa) and β-actin (42 kDa) for C33a and CaSki and E-cadherin (110 kDa) for CaSki are shown on the left. Bar charts on the right show the Western blot quantification of these protein expression levels following treatment. Values are expressed as a fold change of control (n = 3). ( C ) RT-qPCR analysis of relative ZO-1 (C33a and CaSki) and E-cadherin (CaSki) mRNA expression levels following 48 h treatment. Values are expressed as fold change of control values. Data is presented as mean ± SD (n = 3). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Bold asterisk above a single sample bar indicates the significant difference of that sample from the rest of the treatments.

Article Snippet: According to human protein atlas [ ], the C33a cell line exhibits no expression of CDH1 gene, which encodes E-cadherin; however, mass spectrometry proteomics data could further confirm its protein expression.

Techniques: Expressing, Immunofluorescence, Staining, Microscopy, Fluorescence, Immunocytochemistry, Control, Western Blot, Quantitative RT-PCR

Journal: Current Issues in Molecular Biology

Article Title: The Therapeutic Effect of EZH2 Inhibitors in Targeting Human Papillomavirus Associated Cervical Cancer

doi: 10.3390/cimb47120990

Figure Lengend Snippet: Immunohistochemical staining results of EZH2, p53, ZO-1, β-catenin, and HPV16 E6 from CAM assay tissue sections after 48 h treatment with control (DMSO 0.1%), cisplatin, and EPZ6438. Immunohistochemical staining of ( A ) C33a and ( B ) CaSki for expression of EZH2, p53, ZO-1, β-catenin, and HPV16 E6 from the CAM tissue model (n = 1). Scale = 50 µm. Chorionic (CE) and allantoic (AE) epithelial layers with sub-epithelial capillary network (SEC), tumour cells (TC), mesoderm (M), and blood vessels (BV) are displayed.

Article Snippet: According to human protein atlas [ ], the C33a cell line exhibits no expression of CDH1 gene, which encodes E-cadherin; however, mass spectrometry proteomics data could further confirm its protein expression.

Techniques: Immunohistochemical staining, Staining, Chick Chorioallantoic Membrane Assay, Control, Expressing