c33a cells  (ATCC)

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Expression of the EGFR/HER-receptor family in human lung cancer and a cervix cancer cell-line. RT-PCR was performed with 12 non-small cell lung cancer (NSCLC) cell lines and one cervical cancer cell line (C33A) to evaluate the expression of EGFR/HER2, HER3, and HER4-receptor mRNA. β-Actin was used as a loading control. Primers used are shown in , and experimental conditions are as described in Materials and Methods. This experiment is representative of 2 others. The original western blot images can be found in .

Article Snippet: As a positive control [ , , ], the cervical cancer cell line C33A was obtained from ATCC.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Protein expression of HER4, GRPR, and NRG1 in 9 NSCLC cell lines as well as the cervix cancer cell line C33A. ( A ) Representative blot of HER4, GRPR, NRG1, and Tubulin. ( B ) Relative protein expression of HER4, GRPR, and NRG1. The cell lysates were subjected to Western blotting and analyzed using anti-HER4, anti-GRPR, anti-NRG1, and, as a loading control, anti-tubulin. Bands were visualized using chemiluminescence. Results are expressed as the ratio of the protein expression compared to tubulin. This experiment is representative of 2 others. Abbreviations: GRPR, Gastrin-Releasing Peptide Receptor; NRG1, Neuregulin-1. The original western blot images can be found in .

Article Snippet: As a positive control [ , , ], the cervical cancer cell line C33A was obtained from ATCC.

Techniques: Expressing, Western Blot, Control

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Expression of the Neuregulin family in human NSCLC cell lines, one cervical cancer cell line, one prostate cancer cell line, and one breast cancer cell line. PCR was performed with 9 human lung cancer cell lines, one cervical cancer cell line (C33A), and one prostate (Prostate-Z) and breast cancer cell line (MCF-7) to evaluate the expression of Neuregulin mRNA. β-Actin was used as a loading control. Primers used are shown in , and experimental conditions are as described in Materials and Methods. This experiment is representative of 2 others. Abbreviation: NRG, Neuregulin. The original western blot images can be found in .

Article Snippet: As a positive control [ , , ], the cervical cancer cell line C33A was obtained from ATCC.

Techniques: Expressing, Control, Western Blot

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Expression of HER4 Isoforms in human NSCL cancer cell lines. RT-PCR was performed with 8 human lung cancer cell lines, one cervix cancer cell line (C33A), brain and cerebellum to evaluate the expression of HER4 Isoforms (JM-a, JM-b, CYT1, and CYT2) mRNA. β-Actin was used as a loading control. Primers used are shown in , and experimental conditions are as described in Materials and Methods. This experiment is representative of 2 others. Abbreviations: CYT, cytosolic C-terminus; JM, extracellular juxtamembrane region. JM-a, JM-b, CYT1, and CYT2 are HER4 splice variants. The original western blot images can be found in .

Article Snippet: As a positive control [ , , ], the cervical cancer cell line C33A was obtained from ATCC.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot